Creation and Use of Infectious Virus Vector
http://www.stewartlab.net/Protocols/Making_Virus_1...
(Stewart Lab, Washington University at St. Louis)
Extraction of Geminiviral DNA from a Highly Mucilaginous Plant Protocol http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb18/R00-056.pdf
Protocol for the extraction of geminiviral DNA from a highly mucilaginous plant (Abelmoschus esculentus). Includes: Extraction; Alkali lysis; Cloning.
Generation of recombinant baculoviruses by co-transfection (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
http://www.its.caltech.edu/%7Ebjorker/Baculovirus-...
For co-transfection prepare at least 10mg of highly purified plasmid DNA. Spodoptera frugiperda cells are sensitive to some contaminants found in crude plasmid preparations, which cannot be removed by phenol/chloroform extraction or ethanol precipitation.
Gradient Purification of AT2 Inactivated HIV-1 Protocol http://ubik.microbiol.washington.edu/protocols/bl3/at2.htm
Protocol for gradient purification of AT2 inactivated HIV-1.
HIV-1 p24 Antigen Capture ELISA Protocol http://ubik.microbiol.washington.edu/protocols/bl3/p24elisa.htm
Protocol for HIV-1 p24 antigen capture ELISA.
Information Resources for Adjuvants and Antibody Production
http://www.nal.usda.gov/awic/pubs/antibody/
Article explaining the function of adjuvants, providing guidelines for their use, particularly Freund's adjuvants, and introducing some alternatives that are commercially available.
Immunohistochemistry: In Situ Hybridization
http://www.immunoportal.com/
Includes details of immunoenzyme techniques, immunofluorescence and in situ hybridizations, with a photograph gallery and discussion board.
Purification of Bacteriophage lambda Arms: Centrifugation through Sucrose Density Gradients Protocol
http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3990
Protocol is used to prepare the arms of any bacteriophage {lambda} vector.
Purification of Bacteriophage lambda Particles by Isopycnic Centrifugation through CsCl Gradients
http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3968
Isopycnic centrifugation through CsCl gradients is used to prepare infectious bacteriophage {lambda} particles of the highest purity that are essentially free of contaminating bacterial nucleic acids.
Purification of Recombinant Adeno-Associated Virus (rAAV) and Parovirus Protocol http://www.axis-shield.com/densityhome/optiprep/M04.pdf
Protocol for the purification of recombinant adeno-associated virus (rAAV) and parvovirus in pre-formed iodixanol gradients.
Retroviral infection of primary human keratinocytes (PHKs)
http://www.methodbook.net/cellcult/infectn.html
(Matt Lewis, Department of Pathology, University of Liverpool)
SDS-PAGE Sample Preparation of Adenovirus Infected Cells Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1752
Protocol for SDS-PAGE sample preparation of adenovirus infected cells.
TEV Purification Protocol http://openwetware.org/wiki/Keating:Experimental_Protocols:TEV_purification
Protocol for TEV purification.
Titration of Retrovirus Protocol
http://www.methodbook.net/virus/titratn.html
Protocol for the titration of retrovirus.
Viral Detection and Concentration in Food http://members.ift.org/NR/rdonlyres/69B323CE-B14C-4DD6-8F56-B25898C9EFB0/0/crfsfsv01n2p082085ms20010312.pdf
Methods and information related to viral detection and concentration in food. Includes: Nucleic acid amplification methods; Recent progress in polymerase chain reaction detection; Detection of human enteric viruses in non-shellfish food; Choice of primers for the detection of human caliciviruses in foods; Biosensors and microarray detection; Alternative confirmation methods.
http://www.bio.com/protocolstools/protocol.jhtml?id=p617
Protocol describes the production of a dot blot membrane spotted with RNA isolated from virus particles.
Virus detection protocols for west nile virus in vertebrate and ...
www.ncbi.nlm.nih.gov/pubmed/12904372
Protocols to detect the presence of WNV have been refined since 1999 for ... Polymerase Chain Reaction/methods; RNA, Viral/isolation & purification ...
Virological Laboratory Techniques & Resources
(Source: All the Virology on the WWW)
BioGuide - PCR - general information about the polymerase chain reaction including primer design, hot-start, etc.
BioProtocol, Inc. - This site contains a large collection of high quality protocols for the life science researcher. These protocols include offerings from the virology field of study. The company has a highly reputable editorial board to oversee the quality of its protocol collection as well as an editorial staff committed to procuring and producing the highest quality protocols available on-line.
Detailed protocol for gold labeling proteins for electron microscopy. This protocol describes a modified antibody-protein A-gold technique that eliminates the aggregation problem that frequently occurs with the usual procedure.
Cell Biology Laboratory Manual by Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College, St. Peter, MN.
SPOTTER: a new tool to measure viral plaques. The plaque assay is a classical virological technique, originally developed for bacteriophages by Felix d'Herelle (1917). Dulbecco and Vogt subsequently adapted the assay for mammalian viruses, and it is still widely used for virus isolation and purification, and to determine viral titers. The plaque assay is also frequently used to study in vitro growth properties of mutant viruses. A freeware program,called SPOTTER, operating under Win95 and up, allows convenient and precise analysis of large numbers of plaques.
Protocol On-Line - an exhaustive collection of methods and protocols for molecular and cellular biology from academic and comercial laboratories. There is also a Q&A sections that may be helpful for solving lab technique problems.
Comprehensive Protocol Collection - This resource attempts to offer a comprehensive collection of molecular biology protocols. The site links to protocols either maintained directly by the Ambros Lab at Dartmouth or at other locations around the world.
The HHMI Virtual Lab: This interactive laboratory, developed with Shockwave, demonstrates how an enzyme-linked immunosorbent assay (ELISA) is carried out and some of the experimental problems that may be encountered. A free Shockwave plugin is required.
Molecular Biology Protocols - a General Resource from the NOAA of Molecular Methods, Manuals, and some great Links.
PCR in situ: New Technologies with Single Cell Resolution for the Detection and Investigation of Viral Latency and Persistance" from the University of Minnesota
PCR Jump Station - This site attempts to provide scientists with a comprehensive directory for the polymerase chain reaction (PCR) and related techniques.
Labexplorer.com is a new web site written by clinical laboratorians for clinical laboratorians. Designed to be a comprehensive resource for those interested in clinical laboratory practices, the site provides a mechanism of communication between laboratorians, access to experts in a variety of clinical fields, job and career information, and comprehensive links to laboratory sites, diagnostic companies, organizations & interesting topics.
Molecular Biology Protocols on the Web
a great collection of protocol links from the publishers of Molecular Biology Today.
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