Protein purification is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterisation of the function, structure and interactions of the protein of interest. The starting material is usually a biological tissue or a microbial culture. The various steps in the purification process may free the protein from a matrix that confines it, separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps exploit differences in protein size, physico-chemical properties and binding affinity.
1. ALKYL Aspartamide HIC Columns
2. Comparison of affinity tags for protein purification.
5. Green Fluorescent Protein Isolation (Gary J. Lindquester)
6. GST Fusion Protein Preparation
7. GST Fusion Protein Purification from Yeast
8. GST-Fusion Protein Purification
9. HeLa Cell Nuclei Preparation (John Garland, The Biochemistry and Molecular Biology Department of The James H. Quillen College of Medicine in Johnson City, TN)
10. HILIC and SEC Columns PolySULFOETHYL and PolyHYDROXYETHYL Columns
11. Isolation of Protein from Tissue (Reddy research laboratory, Neurological Sciences Institute, Oregon Health & Science University)
13. Making Conductivity Curves (Waters Lab)
14. PI-PLC Purification (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
15. Polyaspartic Acid Weak Cation Exchange Columns
16. PolyGLYCOPLEX Complex Carbohydrates Columns
17. PolySULFOETHYL Aspartamide Strong Cation Exchange Columns
18. PolyWAX LP Weak Anion Exchange Columns
19. Preparation of Tonsil Lysate (Springer Lab, Harvard University)
20. Protein Extraction (Frank Bottone Jr.)
21. Protein Extraction from Tissues (Reddy research laboratory, Neurological Sciences Institute, Oregon Health & Science University)
Protein Isolation Protocols (Lamond Lab, University of Dundee)
22. Protein Isolation using Trizol (Frank Bottone Jr.)
27. Protein Purification (handbook)
28. Protein Purification (Michael Blaber)
29. Protein Purification (Michael Blaber)
30. Protein Purification (Michael Blaber)
31. protein purification (Pictures)
32. Protein Purification and Analysis Protocols and Applications Guide (Promega)
33. Protein Purification on 1G3 Column (Bjorkman Group, Howard Hughes Medical
34. Protein Purification Protocols (The Protein Expression and Purification Facility, EMBL)
35. Protein Sample Preparation & Protein Purification (Invitrogen)
36. Purificaiton of Protein by Affinity Chromatography (Krause Lab)
37. Purification of GST Fused Proteins
38. Purification of GST Fused Proteins
39. Purification of His-tagged proteins from Sf9 cells under native conditions
40. Purification of Proteins (NCBI)
41. Purification Scheme for Ubiquitin Expression Lysate (Sosnick Lab, University of Chicago)
42. Purification of Maltose Binding Proteins Fused Proteins
43. Purification of MBP (maLTose-Binding Proteins) Fused Proteins (Bowtell Lab
44. Removal of Nucleic Acids from Protein (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
45. RNA and protein extraction from the same tissue (Ivan J Delgado)
47. Small Scale MBP-fusion Protein Purification (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
48. Subcellular Fractionation of Proteins (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
49. Sucrose Density Gradient Fractionation of Yeast Membranes (Dohlman Lab)
Test Tube for Ion Exchange Chromatography (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
50. The Protein Purification Facility (Dr. Mario Lebendiker)
51. Tips for acid elution of peptides (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
52. Whole Cell Protein Extracts (Morrisey Lab, University of Pennsylvania)
This is for both cytoplasmic and nuclear proteins.
Protein Purification - How to Purify Proteins.
Suggested Reading
Protein Purification HandbookWhat is Biotechnology?Protein Engineering MethodsWhat is a Protein?What are Enzymes?
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