Protocols online--(B)

Websource bioprotocols (in alphabeta)

BIELSCHOWSKY METHOD
Bile acids
BioAnalyzer
Bioanalyzer
Biochemical Techniques - Bowtell (ORIGIN and UPDATE)
Biological Educational Resources
Biomedical Job Opportunities
Biomek configuration for single stranded DNA isolation
BioRad Bradford protein assay
Biotech Company AddressesBioanalyzer
BLAST
BLAST
Blocking non-specific antigen
Blood cell fractionation (white blood cell isolation)
Blood sampling from animals
Bowtell Lab Manual v31.8.95 (ORIGIN and UPDATE)
B-PER Bacterial Protein extraction reagent (Pierce)
Bradford quantification of protein concentration
BrdU Staining Histological Sections
Brendan Babb's BBHelp - Zappe Techniques Course Files (ORIGIN)
Brew Recipe
Bst-catalyzed radiolabeled DNA sequencing
Buffers
Buffers (Gerard R. Lazo)

Protocols online--(B)

Websource bioprotocols (in alphabeta)

B. Concentration of DNA by ethanol precipitation
B. Midiprep double-stranded DNA isolation
B. Nebulization
B. Purification of PCR fragments for cloning
B. Radiolabeled sequencing gel preparation, loading, and electrophoresis
B95-8 EBV virus Prep.pdf
BACs@CalTech - M. Simon (ORIGIN)
BACs@TAMU - B. Creel (ORIGIN)
Bacterial artificial chromosomes
Bacterial cell culture
Bacterial Colony PCR: Allows quick identification of correct clones if primers exist…
Bacterial transformation
Bacterial Transformation and Transfection
Bacteroid Isolation
Basic knowledge of microarray.
Basic skills for animal experiments
BCIP
Belcher/Knight: Electrocompetent Cells
Beta-galactosidase assay
Beta-Galactosidase Assay (A better Miller)
Bgal Staining Whole Mount
BIELSCHOWSKY METHOD

Protocols online--(A)

Websource bioprotocols (in alphabeta)

Antibiotics (Gerard R. Lazo)Buffer Listing (Salmon Lab.)
Antibody Affinity Purification (GST Column)
Antibody purification
Antibody storage and handling
Antigen retrieval
Aorta ring assay
AP-BCIP/NBT Immunostaining
Apoptosis and related protocols
APPENDIX
Arabidopsis cell suspension and root liquid culture
Arabidopsis DNA Purification from Nuclei
Arabidopsis gDNA isolation
Arabidopsis mutants
Arabidopsis root culture
Arabidopsis Seed Collection
Arabidopsis Transformation
Arabidopsis Transformation by Vacuum Infiltration
Arabidopsis Vacuum Infiltration
Assaying mating
Assembly pcr
Asymmetric PCR for ssDNA Production
Automated Slide Processor (ASP) Version for hybridization.
Autoradiography

Protocols online--(A)

Websource bioprotocols (in alphabeta)

Alkaline Lysis DNA Purifications from Bacteria
alkaline phosphatase
ALKALINE PHOSPHATASE
ALKALINE PHOSPHATASE
ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
Alkaline Southern Blotting Procedure: Kim Marshall
Alm:Agarose gel electrophoresis
Alm:Agarose gel electrophoresis
Alm:Agarose gel electrophoresis
Alpha Project
Amino acids
Amino-allyl labeling
Ammonium Sulfate Saturation Table
Analysis of RNA quality
Anatomy and physiology of animals
AND ALWAYS REMEMBER
Animal models for depression-like and anxiety-like behavior
Anneal Extend PCR Protocol - for linking 2 pieces of DNA
Annealing and primer extension
Annealing primers
Antibiotic Solutions
Antibiotics

Protocols online--(A)

Web source bioprotocols (in alphabeta)

Advanced lab skills (2)
Affinity chromatography
Affinity column preparation and Immunoprecipitation
Affinity purification of Antibodies
Affymetrix DNA labelling for gene expression arrays
Affymetrix One Cycle Eukaryotic Gene Expression Sample Processing
Affymetrix Target Hybridization
Affymetrix Two Cycle Eukaryotic Gene Expression Sample Processing
AFLP (Amplified Fragment Length Polymorphisms)
Agarose Gel Electrophoresis
Agarose gel electrophoresis
Agarose gel electrophoresis
Agarose gel electrophoresis
Agarose gel electrophoresis
Agarose Gel Electrophoresis of DNA
Agarose Pads for Microscopy
Agrobacterium-mediated Transformation of Cotton
ALCIAN BLUE TECHNIQUE
ALCIAN BLUE TECHNIQUE
Alfalfa Leaf Disc Transformation
Alkali Blotting of DNA for Hybridization
ALKALINE CONGO RED TECHNIQUE
ALKALINE CONGO RED TECHNIQUE

Protocols online--(A)

Web Source Bioprotocols--in alphabeta

A rapid, reliable method for preparing somatic chromosomes of rice
A simple screning method for transgenic rice tissue based on PCR
A. Large scale double-stranded DNA isolation
A. Phenol extraction of DNA samples
A. Polymerase Chain Reaction (PCR)
A. Sonication
A. Thaliana Topics
ABC Random AmplificationAlcohol Precipitation of DNAAML sample DNA preparation
ABI 373 Sequencers
ABI Catalyst 800
Acetic acid-Urea PAGE of Protein
Acetic acid-Urea PAGE of Protein
ACETYL CHOLINESTERASE
ACETYL CHOLINESTERASE
ACID PHOSPHATASE
ACID PHOSPHATASE
ACID PICRO MALLORY
ACID PICRO MALLORY
Acrylamide gels
ADENOSINE TRIPHOSPHATASE (ATPASE)
ADENOSINE TRIPHOSPHATASE (ATPASE)
Advanced lab skills

Buffers and solutions--3

1 M (NH4)2SO4
1 M CaCl2
1 M Dithiothreitol (DTT)
1 M Glycine-NaOH Buffer (pH 10)
1 M KCl
1 M Magnesium Acetate
1 M MgCl2
1 M MgSO4
1 M MnCl2
1 M NaH2PO4
1 M Phosphoric Acid
1 M Sodium Citrate
1 M Sodium-Phosphate Buffer
1 M Spermidine
1 M Tris-HCl
1 M ZnCl2
20% Maltose and 1 M Maltose
0.1 M HEPES/ 1 M HEPES
0.5 M EGTA
5 M NaCl
5 M NaOH
10 M Ammonium Acetate
10 M LiCl
0.5 M Tris-Phosphate (pH 6-8)
2.5 M CaCl2
3 M Sodium Acetate (pH 5.2)
1 M PIPES (pH 6.8)
3 M Sodium Acetate (pH 7.0)
1 M Potassium Acetate (pH 7.5)
0.5 M EDTA (pH 8.0)

Buffers and solutions--2

2-D Gel Electrophoresis of Protein
2-D Gel Electrophoresis of Protein
2D Page-Two - Dimensional Polyacrylamide Gel Electrophoresis
2-Mereaptoethanol (BME)
3% NiCl2
3-AMINOPROPYLTRIETHOXYSILANE TREATED SLIDES
3-AMINOPROPYLTRIETHOXYSILANE TREATED SLIDES
3'-end Labeling of DNA with Klenow Fragment
3'-end Labeling of Oligonucleotides with Terminal Transferase
30% Acrylamide:Bis Solution
4% PARAFORMALDEHYDE
4% PARAFORMALDEHYDE
40% Acrylamide:Bis Solution
5% BromoPhenol Blue
5% Xylene Cyanol
50% Glycerol
50% Nonidet P40
50% Triton X-100
5'-end Labeling of DNA with T4 Polynucleotide Kinase
96-Well Format Yeast Protein Purification Protocol
96-well Flow Setup Record (PDF)
1 M Tricine-NaOH (pH 8.0-7.0)
1 M Tris-Acetate (pH 8.0-7.0)
0.1 M ATP

Buffers and solutions--1

1% Acetylated BSA (10 mg/ml)
1% Coomassie Brilliant Blue R
1% Gelatin
1% Pepsin
1% Toluidine Blue
1. Terminator Reaction Clean-Up via Centri-Sep Columns
10 mM Phenylmethylsulfonyl Fluoride (PMSF)
10 ug/ul Glycogen
10% Ammonium Persulfate
10% Blocking Reagent
10% FORMAL SALINE
10% FORMAL SALINE
10% Sarkosyl
10% Sodium Dodecyl Sulfate (SDS)
10% Tween-20
100 mM dNTPs
100 mM NTPs
100 mM rATP
100% Denhardt's Solution
2 X BES-Buffered Saline
2 X HEPES-Buffered Saline
2 X YT Medium
20 X SSC
20 X SSPE