Friday, February 9, 2018

Molecular biology Protocols

  • One-Tube Isolation of DNA from Mouse Tails

    • Michael R. Green
      •  
    • and Joseph Sambrook
    Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot093534

  • Buffers

    • Michael R. Green
      •  
    • and Joseph Sambrook
    Cold Spring Harb Protoc 2018; doi:10.1101/pdb.top098210

  • Caring for Escherichia coli

    • Michael R. Green
      •  
    • and Joseph Sambrook
    Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot101337

  • Protein Immunodepletion and Complementation in Xenopus laevis Egg Extracts

    • Christopher Jenness, 
    • David J. Wynne, 
    • and Hironori Funabiki
    Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot097113

  • Analysis of Mitotic Checkpoint Function in Xenopus Egg Extracts

    • Yinghui Mao
    Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot099853

  • Transformation of Schizosaccharomyces pombe in a 96-Well Format

    • Assen Roguev, 
    • Jiewei Xu, 
    • and Nevan J. Krogan
    Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot091942

  • DNA Preparation from Schizosaccharomyces pombe

    • Assen Roguev, 
    • Jiewei Xu, 
    • and Nevan J. Krogan
    Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot091959

  • Preparation of Plasmid DNA by Alkaline Lysis with Sodium Dodecyl Sulfate: Maxipreps

    • Michael R. Green
      •  
    • and Joseph Sambrook
    Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot093351

  • A Single-Step Method for the Simultaneous Preparation of DNA, RNA, and Protein from Cells and Tissues

    • Michael R. Green
      •  
    • and Joseph Sambrook
    Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot093500

  • Gateway Recombinational Cloning

    • John S. Reece-Hoyes
      •  
    • and Albertha J.M. Walhout
    Cold Spring Harb Protoc 2018; doi:10.1101/pdb.top094912
    • Abstract
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    • Full Text
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    • Full Text (PDF)

      • Propagating Gateway Vectors

        • John S. Reece-Hoyes
          •  
        • and Albertha J.M. Walhout
        Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot094920

      • Generating an Open Reading Frame (ORF) Entry Clone and Destination Clone

        • John S. Reece-Hoyes
          •  
        • and Albertha J.M. Walhout
        Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot094938

      • Using Multisite LR Cloning to Generate a Destination Clone

        • John S. Reece-Hoyes
          •  
        • and Albertha J.M. Walhout
        Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot094946

      • Preparation of DNA from Embryonic Stem Cells or Other Cultured Cells

        • Richard Behringer, 
        • Marina Gertsenstein, 
        • Kristina Vintersten Nagy, 
        • and Andras Nagy
        Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot092718

      • Analysis of DNA Methylation in Mammalian Cells

        • Paul M. Lizardi, 
        • Qin Yan, 
        • and Narendra Wajapeyee
        Cold Spring Harb Protoc 2017; doi:10.1101/pdb.top094821

      • Methylation-Specific Polymerase Chain Reaction (PCR) for Gene-Specific DNA Methylation Detection

        • Paul M. Lizardi, 
        • Qin Yan, 
        • and Narendra Wajapeyee
        Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot094847

      • Methyl-Cytosine-Based Immunoprecipitation for DNA Methylation Analysis

        • Paul M. Lizardi, 
        • Qin Yan, 
        • and Narendra Wajapeyee
        Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot094854

      • High-Throughput Deep Sequencing for Mapping Mammalian DNA Methylation

        • Paul M. Lizardi, 
        • Qin Yan, 
        • and Narendra Wajapeyee
        Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot094862

      • Preparation of Single-Stranded Bacteriophage M13 DNA by Precipitation with Polyethylene Glycol

        • Michael R. Green
          •  
        • and Joseph Sambrook
        Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot093419

      • Growing Bacteriophage M13 in Liquid Culture

        • Michael R. Green
          •  
        • and Joseph Sambrook
        Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot093435

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