Gathering of Bioprotocols Online (1)

This site (www.ebiotek.com) provides hundreds of regular protocols,tutorials,pathways,and databases in life science. In addition, it hosts a biology blog where you can share your own resource with the community and get news from this open biology socical network. Thanks in advance for your sincere contribution.

3H-Thymidine Uptake by Cultured Cells

Affinity magnetic separation (AMS) and immunomagnetic separation (IMS)

All Mouse Models Federated Database

Animal cloning and food safety 

Animal models Protocols    

Animal Search Engines      

Application Guides and Protocols (Nest Group)

Arabidopsis Protocols  

Aseptic Technique  

B cell and T cell Isolation         

C. Elegans Genetic Protocols   

C. Elegans: Apoptosis Assays  

C. Elegans: Embryonic cell culture 

C. Elegans: Protein-protein and protein-DNA interactions    

C. Elegans: Visualizing cells and their components 

Cell Adhesion  

Chemotaxis Assay       

ChIP Assay Protocols  

Chorioallantoic Membrane Vascular Assay  

Chromosome In Situ Hybridization  

Chuck Perou’s Tumor mRNA Isolation Protocol      

Citrate test

Cleaning of PCR product          

Cleaning of the sequencing reaction products by precipitation

Cloning

Collection of Materials for DNA Analysis     

Collection of Materials for DNA Analysis

Comet Assay   

Competitive ELISA Protocols   

Crossover PCR      

Culture of Astrocytes and other neural cells

Culturing Human Embryonic Stem Cells in Feeder-Free Conditions

Cytokine Assay Protocols         

Detailed Troubleshooting Tips for PCR       

DNA Analysis by Flow Cytometry   

DNA and Evidence Collection Principles     

DNA FISH Protocol       4733

DNA Labeling Protocols     

DNA Methods （3）    

DNA Nanoparticles Protocols   

DNA Quantification      

DNA sample Collection procedures.      

Electrophoresis Protocols (1-10)           

Electrophoresis Protocols (11-26)   

Elegans Immunohistochemistry      

ELISPOT Protocol       

End Labeling Protocols (for DNA & RNA)          

Enzyme Assay Protocols (1-7)       

Enzyme Assay（15-21）         

Enzyme Assay（22-28）   

Enzyme Assay（29-35)      

Enzyme Assay（36-42）         

Enzyme Assay（43-47）         

Enzyme Assay（8-14）     

Epigenetics Protocols  

Establishment and characterization of human embryonic stem cell line     

FACS: Cell Cycle Assay Protocols        

Fatty Acid Oxidation Assay

FCM ：PARP PE        

FCM: CaspaTag Caspase Activity Protocol       
Antibody cleanup         

FCM: FASC Analysis of Cell Cycle using BrdU and PI   

FCM: Immunofluorescent Staining of Rat Thymocytes        

FCM: Sodium and Potassium Analysis       

Fixation & Tissue process Protocol       

Fluorescence in situ hybridization (FISH)          

Full protocol for shRNA Transfection          

General PCR introduction        

Genetic Analyses   

Genomic Tip protocol   

Glucose tolerance test and fasting insulin test   

Green fluorescent protein         

Grocott's Methenamine Silver (GMS) stain and troubleshooting       

Guidelines for Techniques with Rodents

H & E Stain Troubleshooting          

H and E Stain (Full Protocol)          

H and E Stain Protocols     

Handbook for DNA isolation, RAPD-PCR and PCR-RFLP   

Hop DNA Extraction Protocol         

Hot-start PCR  

HPLC Troubleshooting 

Immunohistochemistry Stain for Frozen tissue        

Immunohistochemistry: Retrievals        

Immunoperoxidase staining of culture cells       

IN VITRO TRANSCRIPTION OF RNA       

Introduction to HPLC   

Introduction to PCR      

Kinase end-labeling of DNA      

Lab. Safety in Life Science       

Lipid Monolayer Assay Protocol

Lipid-Protein Overlay Assay (Fat Blotting)   

Long Term Storage of Bacterial Strains 

Matrigel invasion assays    

membrane fractionation

Membrane Potential Analysis by Flow Cytometry    

Mesoplasma florum: Tn5 Transposase 

Mesoplasma florum:Electroporation

Mesoplasma florum:Genomic DNA 

Mesoplasma florum:Inverse PCR Transposon location