Gathering of bioprotocols online (2)

Mesoplasma florum:restriction enzyme tests     

Methods to determine the size of an object in microns        

Methylene Blue DNA staining protocol   Administrator

Microscope Techniques (1)       

Microscope Techniques (2) 

Modified Immunohistochemical Protocol for Paraffin Embedded Sections

Mouse Aorta Ring Assay    

mRNA Isolation Protocol    

mRNA Purification 

Multiple and Portable ELISA (M&P ELISA)       

Murine Models of Human Breast Cancer (A)     

Murine Models of Human Breast Cancer (B)     

Mutagenesis    

Neural Stem Cell Protocols

Neural Stem Cell Protocols 2   

NIH Protocols for Immunohistochemistry          

Nucleic Acids Research Methods (2)    

Oxidase test    

PCR analysis of DNA from Laser Microdissected Samples        

PCR Animations          

PCR Application Manual           

PCR Based Molecular Cloning       

PCR Elisa        

PCR products cloning        

PCR Resource Online        

PCR RFLP Administrator   

PCR SSCP Administrator   

PCR, RT PCR and Real Time PCR Tutorials    

Phosphate Assay – Lipids  

Pipette Calibration       

Plasmid Protocols--(31-37)       

Polar Lipid Extraction   

Polysaccharide sequencing

Preventing primer-dimer formation        

Probe Prep, 32P End-Labeled Probes  

Protein Protein Crosslinking Protocols        

Protein Purification and Identification Protocols       

Protocol for Bioanalyzer RNA nano chip      

PROTOCOL FOR SINGLE CELL mRNA AMPLIFICATION       

Protocols for diabetes research

Protocols for In Vitro Translation          

Protocols for induced pluripotent stem cells

Protocols for SiRNA     

Pulse-chase protein production      

Radioimmunoassay--RIA Protocols      

Real Time PCR Administrator   

Reverse transfection    

Reverse Transfection Protocols      

RNA FISH Protocols          

RNA Isolation        

RNA MicroArray Protocol         

RT-PCR Protocols       

Sample protocol for material to be used in DNA barcoding        

SELEX—in vitro selection        

shRNA Protocols         

shRNA Protocols (2)          

Subculturing Human Umbilical Vein Endothelial Cells (HUVEC)       

Tail Vein Injection Techniques         

Telomere and telomerase assay           

Telomere and telomerase protocols      

Thiobarbituric acid reactive substances (TBARS) Assay

Titration of retrovirus    

Troubleshooting Guide for Immunohistochemistry   

Troubleshooting Guide: Western Blotting    

TROUBLESHOOTING TIPS – Western Blotting           

Troubleshooting: RNA Isolation      

Variants of PCR (2)      

Various Procedures in Model Building using Coot          

Voges–Proskauer test  

Weil’s myelin stain       

Western blot analysis using the Odyssey Infrared Imaging System  

Western Blot Protocol        

Yeast Cellular Biology  

Yeast Culture and Storage 

Yeast DNA and RNA Methods  

Yeast Genetics

Yeast PCR Protocols   

Yeast Protein Methods       

Yeast Transformation   

Yeast Two-Hybrid System

Gathering of Bioprotocols Online (1)

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3H-Thymidine Uptake by Cultured Cells

Affinity magnetic separation (AMS) and immunomagnetic separation (IMS)

All Mouse Models Federated Database

Animal cloning and food safety 

Animal models Protocols    

Animal Search Engines      

Application Guides and Protocols (Nest Group)

Arabidopsis Protocols  

Aseptic Technique  

B cell and T cell Isolation         

C. Elegans Genetic Protocols   

C. Elegans: Apoptosis Assays  

C. Elegans: Embryonic cell culture 

C. Elegans: Protein-protein and protein-DNA interactions    

C. Elegans: Visualizing cells and their components 

Cell Adhesion  

Chemotaxis Assay       

ChIP Assay Protocols  

Chorioallantoic Membrane Vascular Assay  

Chromosome In Situ Hybridization  

Chuck Perou’s Tumor mRNA Isolation Protocol      

Citrate test

Cleaning of PCR product          

Cleaning of the sequencing reaction products by precipitation

Cloning

Collection of Materials for DNA Analysis     

Collection of Materials for DNA Analysis

Comet Assay   

Competitive ELISA Protocols   

Crossover PCR      

Culture of Astrocytes and other neural cells

Culturing Human Embryonic Stem Cells in Feeder-Free Conditions

Cytokine Assay Protocols         

Detailed Troubleshooting Tips for PCR       

DNA Analysis by Flow Cytometry   

DNA and Evidence Collection Principles     

DNA FISH Protocol       4733

DNA Labeling Protocols     

DNA Methods （3）    

DNA Nanoparticles Protocols   

DNA Quantification      

DNA sample Collection procedures.      

Electrophoresis Protocols (1-10)           

Electrophoresis Protocols (11-26)   

Elegans Immunohistochemistry      

ELISPOT Protocol       

End Labeling Protocols (for DNA & RNA)          

Enzyme Assay Protocols (1-7)       

Enzyme Assay（15-21）         

Enzyme Assay（22-28）   

Enzyme Assay（29-35)      

Enzyme Assay（36-42）         

Enzyme Assay（43-47）         

Enzyme Assay（8-14）     

Epigenetics Protocols  

Establishment and characterization of human embryonic stem cell line     

FACS: Cell Cycle Assay Protocols        

Fatty Acid Oxidation Assay

FCM ：PARP PE        

FCM: CaspaTag Caspase Activity Protocol       
Antibody cleanup         

FCM: FASC Analysis of Cell Cycle using BrdU and PI   

FCM: Immunofluorescent Staining of Rat Thymocytes        

FCM: Sodium and Potassium Analysis       

Fixation & Tissue process Protocol       

Fluorescence in situ hybridization (FISH)          

Full protocol for shRNA Transfection          

General PCR introduction        

Genetic Analyses   

Genomic Tip protocol   

Glucose tolerance test and fasting insulin test   

Green fluorescent protein         

Grocott's Methenamine Silver (GMS) stain and troubleshooting       

Guidelines for Techniques with Rodents

H & E Stain Troubleshooting          

H and E Stain (Full Protocol)          

H and E Stain Protocols     

Handbook for DNA isolation, RAPD-PCR and PCR-RFLP   

Hop DNA Extraction Protocol         

Hot-start PCR  

HPLC Troubleshooting 

Immunohistochemistry Stain for Frozen tissue        

Immunohistochemistry: Retrievals        

Immunoperoxidase staining of culture cells       

IN VITRO TRANSCRIPTION OF RNA       

Introduction to HPLC   

Introduction to PCR      

Kinase end-labeling of DNA      

Lab. Safety in Life Science       

Lipid Monolayer Assay Protocol

Lipid-Protein Overlay Assay (Fat Blotting)   

Long Term Storage of Bacterial Strains 

Matrigel invasion assays    

membrane fractionation

Membrane Potential Analysis by Flow Cytometry    

Mesoplasma florum: Tn5 Transposase 

Mesoplasma florum:Electroporation

Mesoplasma florum:Genomic DNA 

Mesoplasma florum:Inverse PCR Transposon location