Microsatellite Protocols

What’s microsatellites ?

A DNA sequence from 2 to 6 nucleotides which are tandemly repeated from about 5 to 5,000 times (usual range 20 - 50 repeats). Microsatellites are found at many different loci in the genome, including both coding and non-coding regions. A locus containing a microsatellite is often polymorphic because of variation in the repeat number.

Protocols

Optimization of PCR Protocol in Microsatellite Analysis with ...
Here we present the optimization of PCR conditions for microsatellite analysis. of coniferous trees. The use of touchdown protocol for annealing resulted in ...
pubs.nrc-cnrc.gc.ca/ispmb/ispmb18/R00-053.pdf

Microsatellite Cloning Protocol (June 2001)

DeWoody‘s Microsatellite Cloning Protocol (Spring 2002) ..... Microsatellite clones in the kangaroo rat. Using the protocol described above, ...
www.agriculture.purdue.edu/fnr/html/faculty/DeWoody/DeWoodyweb/pdfs/msatclngprtcl.pdf

How Are Microsatellite Repeats Counted?

How Microsatellite Repeats Are Counted ] [ YSTR Database Allele Frequency ... at http://www.ihwg.org/protocols/hct/HCT-microsatellite-protocol.pdf which may ...
www.contexo.info/DNA_Basics/microsatellite_analysis.htm

MHC MICROSATELLITE TYPING PROTOCOL

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MHC MICROSATELLITE TYPING PROTOCOL. SPECIMEN:. Genomic DNA from any source such as whole blood, peripheral blood lymphocytes, serum, or plasma can be used. ...
www.ihwg.org/protocols/hct/MS.doc

Microsatellites Protocol

In order to identify the microsatellites on the Pacu fish, the following protocol had to be followed. Step 1: Extracting the DNA from the nucleated blood ...
www.woodrow.org/teachers/esi/2002/Biology/Projects/p3/protocol.htm

Microsatellite library protocol

The following protocol uses the microsatellite hybrid capture technique for isolating microsatellite loci [Prochazka 1996, Brown et al., 1995]. ...
hcgs.unh.edu/protocol/msat/

Microsatellite Library Protocol Last Update 11/26/03 D Drown ...
Microsatellite Library Protocol. Last Update 11/26/03 D Drown. Modified from T. Garner Protocol. Outline of Protocol. DNA Extraction (1 overnight [optional] ...
people.bu.edu/pbarber/Web%20Protocols/Microsatellite%20Library.pdf

SOLTIS LAB MICROSATELLITE CAPTURE PROTOCOL

This first step in the microsatellite capture protocol uses restriction enzymes to cut. genomic DNA into smaller fragments. We will be using Sau3A I, ...
www.flmnh.ufl.edu/soltislab/Soltis_SSR_Capture.pdf

Science Links Japan | A Protocol for Efficient Development of MicrosatelliteMarkers.

Title;A Protocol for Efficient Development of Microsatellite Markers. Author;LIAN C(Univ. Tokyo, Asian Natural Environmental Sic. ...
sciencelinks.jp/j-east/article/200414/000020041404A0421669.php

Microsatellite Protocol

Microsatellite Protocol. Preparation of reaction mixture. Dilute primers to 10 µM by adding 200 µl 1x TE buffer (see Table 8-9). Each primer in the Rice Map ...
www.pustaka-deptan.go.id/rkb/knowledgeBank/grcOpsManual/_Microsatellite_Protocol.htm - 50k - Cached - Similar pages

Isolating Microsatelline DNA Loci*

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In theory, this protocol will. work for any eukaryotic organism (i.e, anything with an appreciable number of microsatellite ...
www.uga.edu/srel/DNA_Lab/Glenn&Schable_update2.pdf

Microsatellite Protocol.
Microsatellite Protocol. Prepare gel:. 1. Pour Acrylamide gel (mix up 25 mL Acrylamide solution, 125 uL APS, 17 uL. TEMED) ...
people.bu.edu/pbarber/Web%20Protocols/Microsatellite%20gel%20protocol.pdf -

Fully automated DNA purification and efficient multiplex PCR for ...
concentration do not significantly influence. multiplex PCR of microsatellite loci. Table 1. PCR cycling protocol for microsatellite analysis ...
www1.qiagen.com/literature/qiagennews/0103/1023229_QNews12003_p08_10.pdf

Microsatellite Protocol - 30nov
flanking primers for microsatellite amplification. This protocol greatly improves. enrichment by increasing the efficiency of linker ligation to genomic DNA ...
www9.georgetown.edu/faculty/hamiltm1/Downloads_files/SNXmicro11.pdf

SilkSatDB-A Silkworm Microsatellite Database

Protocol for analysing microsatellites using agarose gel electrophoresis, is a fast and efficient technique for standardising primers and quick polymorphism ...
210.212.212.7:9999/PHP/SILKSAT/index.php?f=protocol_ssr

Rob Toonen's Protocol （for Microsatellite）

Estoup & Turgeon's Microsatellite Protocols （for Microsatellite）

Fisher & Bachmann's Protocol （for Microsatellite）

Theresa M. Culley (DNA Based Methods / Microsatellite)

Dec 30, 2005 ... If you are interested in doing this process yourself, you will need to email Travis for the most current microsatellite development protocol ...
www.biology.uc.edu/faculty/culley/Protocols.htm

NOTES Microsatellite Multiplexing in Fish
Step-by-step protocol for the design and optimization of microsatellite multiplexing (modified from ..... mized microsatellite multiplex protocol presented ...
publish.uwo.ca/~bneff/papers/microsatellite_multiplexing_in_fish.pdf

Insect Microsatellite Database

LINKS. Silkworm microsatellite database · Silkbase · Silkworm science/Sericulum · Bombyx mori genome map information system · Silkworm, Nucleic acid/Amino ...
210.212.212.8/PHP/INSATDB/links.php