Chapter.14. BIOCHEMISTRY
Cell Wall prep from Arabidopsis
GC analysis of alditol acetates
Golgi-Vesicles preparation from pea etiolated hypocotyls
High Voltage Paper Electrophoresis
Sunday, December 21, 2008
Web Links: BIOCHEMISTRY
Web Links: BACTERIA
Chapter.15. BACTERIA
Electro Competent Agrobacterium Cells
Introduction of plasmid into Agrobacterium (method II)
Introduction of plasmid into Agrobacterium (method III)
General Cloning Procedures
Colony Hybridization
Electroporation & Blue/White Selection
Large scale plasmid prep, PEG method
Large scale plasmid prep, Qiagen/CsCl
Ligation Optimization
Plasmid Miniprep
Preparation of competent E. coli cells using CaCl2
Transformation of bacteria
Release of recombinant proteins from bacterial cells using Novagen's Bugbuster
Web Links: Arabidopsis Protocols
Chapter.16. Arabidopsis Protocols
Arabidopsis cell suspension and root liquid culture
Arabidopsis EMS mutagenesis of seeds
Arabidopsis genomic DNA prep-CTAB extraction method
Arabidopsis genomic DNA prep-Proteinase K method
Arabidopsis quick genomic DNA prep for PCR
Arabidopsis seed sterilization
Arabidopsis vacuum infiltration (plant transformation)
Confocal Microscopy- Nuclear Staining
Cross-fertilization of Arabidopsis
Crossing Arabidopsis plants (flower emmansculation and flower preparation for fertilization)
Glucocorticoid (GVG)-inducible promoter system for Arabidopsis
In situ hybridization using radioactive probes
Non radioactive in situ hybridization
Plastic Sectioning- Tissue fixation
Protoplast preparation (plant tissue)
Scanning Electron Microscope- Tissue fixation and dissection
Web Links: Yeast Protocols
Chapter.17. Yeast Protocols
96-Well Format Yeast Protein Purification Protocol
A microarray-based protocol for monitoring the growth of yeast .
A protocol for isolation and visualization of yeast nuclei by scanning electron microscopy (SEM).
Brent Lab Two-Hybrid Information
Chromatin immunoprecipitation protocol for Saccharomyces cerevisiae.
Codon Usage Tables.
Common Access to Biological Resources and Information (CABRI)
Direct PCR from Whole Yeast Cells
DNA Replication Protocols from the Fangman and Brewer Labs,
European announcement of the complete Sequence, April 24, 1996.
FASTA
S. cerevisiae protein structural information
Yeast Maps
Genomic View
Worm-Yeast Protein Comparison
Mammalian Homology to Yeast
Yeast Genome Restriction Analysis
Search Saccharomyces GenBank Sequences
Pairwise Chromosome Similarity View
Gene Disruption via PCR
Confirmation PCR
GeneQuiz - Consortium for large scale sequence analysis.
Gietz Lab's Yeast Transformation Page
Growth Chamber for Time Lapse Imaging of Budding Yeast
GST Fusion Protein Purification from Yeast
INTESTINAL DYSBIOSIS / YEAST PROTOCOL
List of species specific documents from SWISS-PROT.
National Collection of Yeast Cultures (NCYC)
Plasmid Rescue from Yeast from Liquid Culture and Clony Rescue
Preparation of Yeast Cell for Immunoeletron Microscopy
Protocol for Yeast-Based Androgen Receptor Assay ...
Quick Total Yeast Protein Isolation for SDS-PAGE Gels
Saccharomyces Gene Registry at SGD.
Slide Stripping Protocol Agilent Yeast Arrays
Staining of yeast by Rhodamine-Phalloidin/Calcofluor
Sucrose Density Gradient Fractionation of Yeast Membranes
The Definitive Yeast Transformation Homepage from the
The World-Wide Web Virtual Library: Yeast
VectorDB, collected sequences of cloning vectors for use in a variety of organisms.
Yeast Actin Purification protocol
Yeast Cell Cycle Flow Cytometry Protocol
Yeast Cell Micromanipulation, a protocol for making a support rod with fiber optic needle attached.
Yeast Genome Project Information from MIPS.
Yeast Histone Prep (from Jakob Waterborg; .pdf format)
Yeast Immunofluorescence protocol
Yeast Immunofluorescence with Methanol/Acetone Dehydration
Yeast Knockout (YKO) Collection
Yeast Lab Protocols from the Botstein Lab,
Yeast Lab Protocols from the Breeden Lab,
Yeast Lab Protocols from the Gottschling Lab,
Yeast Mitochondrial Protein Database
Yeast Nuclear Extract Prep (small scale)
Yeast Protein Preparation for SDS PAGE and Western (rapid)
Yeast Proteome Database (YPD), accessible only to subscribers.
Yeast ProtoArrayTM Proteome Chips
Yeast Protocols from the Gottschling Lab
Yeast Sequencing at the Sanger Centre.
Yeast Tet-promoters Hughes Collection
Yeast Transposon Insertion Library Collection
Web Links: Biomedicine
Chapter.20. Online Resources and Reference Databases
List A (From: FEROLab)
Journal Search via
Entrez-PubMed at NCBI
Community of Science Database
FHCRC Stockroom (FHCRC only)
Methods & Reagents BIOSCI newsgroup
Technical Tips at BioMedNet
Primer3+ Nucleic Acid Databases
Protein Data Bank at Brookhaven
BioMagResBank Pairwise Alignment Servers
BLAST 2 Sequences against each other
BLOCKS at FHCRC
ClustalW at EMBnet
BOXSHADE for easy viewing
Michigan Tech (Map)
UC Santa Cruz (SAM)
Poisson Probability Calculator
Chapter.20. Online Resources and Reference Databases
List B. (From Biotechniques)
Free eBooks in life science (1-4)
How to make buffers and solutions?
Genetics Education and Handbook
Free Software pDRAW32 to Draw DNA Analysis Charts
Biological Educational Resources
Resources of Medical Biochemistry
Cinema Online, Free Movies in Life Science --- (1) (2) (3)
Hot Links:
HHS.gov
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